Coding

Part:BBa_K2198000:Design

Designed by: Gabriel Dournes   Group: iGEM17_Pasteur_Paris   (2017-10-25)


Naphtalene Dioxygenase SubUnit A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1486
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 675
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence optimized for expression in E.coli and for gene synthesis. It has also a RBS, Histag, TEV site in 5' region and a BamhI site in the 3' region.


Source

Pseudomonas putida bacterial DNA.

References